论文部分内容阅读
为探讨滋养层细胞膜的免疫调节作用及TLX与CD46的关系,我们用超声破碎、差速离心制备滋养层细胞膜(StMPM)。经酶学分析、电镜观察、MHC抗原和β_2微球蛋白测定及与标准抗TLX抗体的反应,证实所分离的膜成份纯度、细胞特异性和抗原性。ELISA和FACS竞争抑制试验表明兔抗MCP对兔抗TLX抗体的竞争抑制率为52.3%;兔抗TLX基本上能阻断TRA-2-10、CB-24(均识别CD46)与淋巴细胞的结合。但反之则不然。说明TLX标准抗体能识别CD46以外的抗原。向培养系统中加入StMPM.能特异地、剂量依赖性地抑制丝列原诱发的淋巴细胞增殖、IL-2与sIL-2R分泌、IL-2R、HLA-DR的表达;抑制混合淋巴细胞培养、B细胞IgG的分泌、蜕膜NK和蜕膜、外周血LAK的杀伤活性,但对外周血NK无抑制作用。核酸杂交显示对IL-2Ra、TCR-βmRNA转录无显著影响,但能促进PBL、JurkatTGF-βmRNA的转录。从分子水平表明滋养层细胞膜具有广泛的免疫调节作用,为滋养层细胞在母胎免疫中的重要作用提供了新的实验依据。
To investigate the immunomodulatory effect of trophoblast membrane and the relationship between TLX and CD46, we prepared the trophoblast cell membrane (StMPM) by sonicating and differential centrifugation. Enzyme-linked immunosorbent assay, electron microscopy, determination of MHC antigen and β_2 microglobulin and reaction with standard anti-TLX antibody confirmed the purity, cell specificity and antigenicity of the separated membrane components. ELISA and FACS competitive inhibition assay showed that the anti-MCP rabbit anti-TLX antibody competitive inhibition rate of 52.3%; rabbit anti-TLX can basically block TRA-2-10, CB-24 (both CD46) and lymphocytes The combination of. But the opposite is not true. TLX standard antibodies can recognize antigen other than CD46. Add StMPM to the culture system. Can specifically and dose-dependently inhibit fibroin-induced lymphocyte proliferation, IL-2 and sIL-2R secretion, IL-2R, HLA-DR expression; inhibit mixed lymphocyte culture, B cell IgG secretion, NK and decidual membrane, peripheral blood LAK cytotoxic activity, but no inhibitory effect on peripheral blood NK. Nucleic acid hybridization showed no significant effect on the transcription of IL-2Ra and TCR-β mRNA, but could promote the transcription of PBL and Jurkat TGF-β mRNA. From the molecular level that the trophoblast membrane has a wide range of immunomodulatory effects for trophoblast cells in the role of maternal-fetal immunity provides a new experimental basis.