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F-box蛋白作为SCF(Skp1-Cullin1-F-box)复合体的成员,参与植物生长发育、细胞分裂及植物激素的响应等调控过程。为深入了解大叶落地生根(Kalanchoe daigremontiana)中胎生苗发育的分子机制,利用RACE-PCR技术从大叶落地生根中克隆了1个新的基因Kd FBX(Gen Bank No.KU740356)。该基因具有328个氨基酸残基,分子量为37.72 k D,等电点为4.4,开放阅读框(open reading frame,ORF)长度为987 bp。其蛋白与亚洲棉(Asiatic cotton)、蓖麻(Ricinus communis)等植物的同源性较高,与拟南芥(Arabidopsis thaliana)的进化关系相对接近。实时荧光定量PCR分析表明,该蛋白基因在大叶落地生根的根中表达量最高,且在渗透胁迫(甘露醇)处理过程中下调表达。本研究首次从大叶落地生根中克隆出Kd FBX基因,为进一步探究该基因的功能提供理论依据。
As a member of the Skf1-Cullin1-F-box complex, F-box protein is involved in the regulation of plant growth and development, cell division and plant hormone response. In order to further understand the molecular mechanism of viviparous development in Kalanchoe daigremontiana, a new gene, Kd FBX (Gen Bank No. KU740356), was cloned from leafy rhizome by RACE-PCR. The gene has 328 amino acid residues, a molecular weight of 37.72 kD, an isoelectric point of 4.4 and an open reading frame (ORF) length of 987 bp. Its protein has high homology with plants such as Asiatic cotton and Ricinus communis, and has relatively close evolutionary relationship with Arabidopsis thaliana. Real-time PCR analysis showed that the expression of this protein was the highest in root of Larix gmelini and down-regulated during osmotic stress (mannitol) treatment. In this study, Kd FBX gene was cloned for the first time from root-leaved roots, providing a theoretical basis for further exploring the function of this gene.