反义EGFR寡核苷酸对AngⅡ促血管平滑肌细胞增殖效应的影响

来源 :心脏杂志 | 被引量 : 0次 | 上传用户：guohaoyan

【摘要】

：

目的探讨脂质体介导的反义表皮生长因子受体（EGFR）寡核苷酸基因转染对血管紧张素Ⅱ（AngⅡ）促大鼠血管平滑肌细胞（VSMC）增殖的影响。方法用EGFR寡核苷酸脂质体复合物转染Sprague-Daw

【作者】

：

朱艳霞王启贤邓东波陈鹤方勤尹小龙

【机构】

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昆明医学院第一附属医院心内科,昆明市延安医院心内科

【出处】

：

心脏杂志

【发表日期】

：

2007年4期

【关键词】

：

反义表皮生长因子受体寡核苷酸血管紧张素Ⅱ 血管平滑肌细胞增殖 antisense sequences of epidermal growth factor

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目的探讨脂质体介导的反义表皮生长因子受体（EGFR）寡核苷酸基因转染对血管紧张素Ⅱ（AngⅡ）促大鼠血管平滑肌细胞（VSMC）增殖的影响。方法用EGFR寡核苷酸脂质体复合物转染Sprague-Dawley大鼠VSMC,通过RT-PCR、Western-Bloting分别检测转染后EGFR mRNA及蛋白的表达情况,用3H-Tdr掺入法检测经EGFR寡核苷酸转染再用AngⅡ刺激VSMC的增殖情况。结果反义EGFR寡核苷酸转染大鼠VSMC后,EGFR mRNA及蛋白的表达较正义组及对照组显著减少（P〈0.01

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反义EGFR寡核苷酸对AngⅡ促血管平滑肌细胞增殖效应的影响

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