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提出了邻氨基酚（ＯＡＰ）－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析法测定人血清甲胎蛋白（α－ＦＰ）的新方法．该方法是将ＨＲＰ催化Ｈ２Ｏ２氧化邻氨基酸的酶催化反应与邻氨基酚的氧化产物在滴汞电极上的还原反应相偶合，在ＢＲ缓冲溶液中，在－０．４３ Ｖ（ｖｓ．ＳＣＥ）左右产生灵敏的极谱波．根据测定标记在甲胎蛋白抗体上的ＨＲＰ的量，求得发生免疫反应的 α－ＦＰ的含量。该方法对甲胎蛋白测定的线性范围为 １． ２５～４００ ｍｇ／Ｌ。用所建立的方法对病人血清样品进行了测定，并与酶联免疫吸附测定光度法（ＥＬＩＳＡ）进行对照，二者相关性很好。
A new method for the determination of alpha-FP in human serum with o-aminophenol (OAP) -H2O2-HRP voltammetric enzyme-linked immunosorbent assay (ELISA) was proposed. The method is that HRP catalyzes the enzymatic reaction of o-amino acid oxidation of H2O2 with the reduction reaction of the oxidation product of o-aminophenol on the drop mercury electrode, and in a BR buffer solution at about -0.43 V (vs.SCE) Produce sensitive polarographic waves. The amount of α-FP in which an immune reaction occurred was determined from the amount of HRP labeled on the α-fetoprotein. The method has a linear range of 1 for alpha-fetoprotein determination. 25 ~ 400 mg / L. The serum samples of the patients were determined by the established method and compared with enzyme-linked immunosorbent assay (ELISA), the correlation between them was very good.