本文报道了用固相放免技术测定IgG类风湿因子的方法和临床测定结果。本法以聚苯乙烯微量细胞培养板为载体,以兔IgG为抗原,以~(125)Ⅰ标记的提纯兔抗人IgG作为抗抗体,检测类风湿因子。30名献血员血清,~(125)Ⅰ标记抗体结合率为0.54～1.74%,平均数为0.90±1.4%。而57例类风湿性关节炎患者为1.39～18.28%,均数为5.57±1.72%,两组比较差别显著(P<0.01)。 This article reports the method and clinical determination of IgG rheumatoid factor by solid phase radioimmunoassay. This method uses polystyrene microplate culture plate as carrier, rabbit IgG as antigen, ~ (125) I labeled purified rabbit anti-human IgG as anti-body to detect rheumatoid factor. Thirty blood donors’ serum and ~ (125) Ⅰ labeled antibody binding rate was 0.54-1.74% with an average of 0.90 ± 1.4%. The 57 cases of rheumatoid arthritis patients were 1.39 ~ 18.28%, the average was 5.57 ± 1.72%, the difference between the two groups was significant (P <0.01).