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采用3,5-二硝基水杨酸(3,5-dinitrosalicylic acid,DNS)比色法和超微细胞化学定位法,研究了果胶酶和纤维素酶在管花肉苁蓉芽管、吸器和侵染结构界面的分泌和分布及对寄主根细胞的作用。结果表明,离体的芽管、吸器均分泌果胶酶和纤维素酶,其含量随着芽管和吸器生长呈增加趋势。在吸器和寄主根的侵染结构界面,侵入细胞也分泌果胶酶和纤维素酶,并在吸器和寄主根相邻的胞间层、寄主细胞壁周边部位分布,导致寄主细胞间连接被解除和细胞的降解。结论:管花肉苁蓉吸器侵入寄主根的过程中有果胶酶和纤维素酶的参与。
3,5-dinitrosalicylic acid (3,5-dinitrosalicylic acid, DNS) colorimetric method and ultra-cytochemistry were used to study the effects of pectinase and cellulase on the germination of tube, Secretion and distribution of infiltrating structure interface and its effect on host root cells. The results showed that the germ tube and the astringents secreted pectinase and cellulase in vitro, and the content of pectinase and cellulase increased with the growth of germ tube and aspirator. Invasive cells also secrete pectinase and cellulase at the interface between the aspirator and the host root at the infection site, and distribute in the periplasm of the aspirate and the host root adjacent to the host cell wall, resulting in the ligation of the host cell being released and Degradation of cells. CONCLUSION: Pectinase and cellulase participate in the process of invading Cistanche tubulosus inhalation to host roots.