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目的为细胞替代治疗缺血性脑损伤选择合适的干细胞。方法将RMNE6细胞植入正常脑和缺血模型大鼠脑(8只)内,通过免疫荧光染色、行为学实验、影像学等方法观察其在脑内的状况,与安慰剂组(8只)及成纤维细胞移植组(8只)比较,观察其对模型动物梗塞体积的影响和模型动物行为学的改善。结果细胞移植后,RMNE6细胞在正常脑和缺血脑内可以存活,但移植有RMNE6细胞的缺血脑内出现肿瘤。细胞移植与否对梗死体积没有影响。细胞移植后,模型动物的行为学也未见明显改善。结论从安全性的角度考虑,RMNE6细胞不适合通过脑内移植治疗缺血性脑损伤,RMNE6细胞还需要改造。
Objective To select appropriate stem cells for cell replacement therapy for ischemic brain injury. Methods RMNE6 cells were implanted into the brains of normal and ischemic rats (8 rats). The immunofluorescence staining, behavioral tests and imaging were used to evaluate the intracerebral function of RMNE6 cells. Compared with the placebo group, And fibroblast transplantation group (8 rats). The effects on the infarct volume of model animals and the improvement of model animal behavior were observed. Results After transplantation, RMNE6 cells could survive in the normal brain and ischemic brain, but tumor appeared in the ischemic brain of RMNE6 cells. Whether or not cell transplantation has no effect on infarct volume. After cell transplantation, no significant improvement in the behavior of model animals. Conclusion From a safety point of view, RMNE6 cells are not suitable for intracerebral transplantation in the treatment of ischemic brain injury, RMNE6 cells also need to be modified.