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多数情况下,羊水中培养的细胞来源于胎儿。偶而,羊水被母体细胞所污染,培养时母体细胞大量增殖,超过了胎儿细胞,导致染色体分析的差错。Ferguson-Smith(1975)曾报道617例羊膜标本中有三例诊断错误,是由于母体细胞污染所致。上述三例细胞核型分析曾认为是正常女胎的核型,但出生后的二个婴儿是正常男孩,另一例是患Down氏综合征的女孩。虽然母体细胞的污染使诊断发生差错是少见的,但应认识其差错的重要性。Pola-ni(1971)和 Ferguson-Smith等(1976)介绍了两种预防方法,两种技术均有其缺点。作者在本文描述的另一种可能检出母体
In most cases, cells cultured in amniotic fluid are derived from the fetus. Occasionally, amniotic fluid is contaminated with maternal cells, maternal cells cultured in large numbers proliferate, exceeding the fetal cells, resulting in chromosome analysis errors. Ferguson-Smith (1975) reported three cases of diagnostic errors in 617 amniotic samples due to maternal cell contamination. The three karyotype analyzes described as karyotypes of normal female fetuses, but two newborn babies are normal boys and the other is girls with Down’s syndrome. Although the diagnosis of maternal cell contamination error is rare, but should recognize the importance of error. Two methods of prevention are described by Pola-ni (1971) and Ferguson-Smith et al. (1976), both of which have their drawbacks. Another possibility that the authors describe here is the detection of maternal