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目的为了解免疫球蛋白G(Ig G)、干扰素γ(INF-γ)的血清含量及CD4+、CD8+T淋巴细胞百分比对不同宫颈病变患者感染高危型人乳头瘤病毒(HR-HPV)转归的影响。方法依据不同检查结果把研究对象分为HPV16阳性正常组、HPV16阳性CINI组、HPV16阳性CINII-III及HPV16阳性SCC组(分期均≤Ib1期)。常规于治疗前行流式细胞仪分别检测CD3+、CD4+、CD8+T细胞百分比,酶联免疫吸附试验(ELISA)法定量检测血清Ig G、INF的含量。12个月后同上述方法重复检查并复查HPV+TCT。依据其不同转归(持续阳性或转阴),统计分析两组之间各因子含量及CD4+/CD8+T细胞百分比变化与组织学及HPV转归之间的关系。结果 1年后不同病变中IFN、CD4+T细胞百分比及CD4+/CD8+T细胞比值在HPV(+)组明显低于HPV(-)组及初测组。而1年后SCC HPV(+)组中Ig G血清含量、CD3+T及CD8+T百分比明显低于初测值及HPV(-)组(P<0.01)。结论机体免疫因子INF血清含量、CD4+T百分比及CD4+/CD8+比值的消长对不同宫颈病变HPV感染的转归起着重要作用。
Objective To investigate the relationship between the serum levels of Ig G, INF-γ and the percentage of CD4 + and CD8 + T lymphocytes in patients with different cervical lesions infected with high-risk human papillomavirus (HR-HPV) The impact of the return. Methods According to the results of different examinations, the subjects were divided into HPV16-positive normal group, HPV16-positive CINI group, HPV16-positive CINII-III and HPV16-positive SCC group (stage ≤Ib1). The percentage of CD3 +, CD4 +, CD8 + T cells were detected by flow cytometry before treatment. The contents of Ig G and INF in serum were detected by enzyme linked immunosorbent assay (ELISA). After 12 months with the above method repeated examination and review of HPV + TCT. According to their different outcomes (persistent positive or negative), the relationship between the changes of the two factors and the percentages of CD4 + / CD8 + T cells and the histology and HPV outcome were statistically analyzed. Results After 1 year, the percentage of IFN, CD4 + T cells and CD4 + / CD8 + T cells in different lesions were significantly lower in HPV (+) group than in HPV (-) group and in the primary test group. After 1 year, the percentage of Ig G serum, CD3 + T and CD8 + T in SCC HPV (+) group was significantly lower than that in initial test and HPV (-) group (P <0.01). Conclusions The increase of serum immunological factors such as INF serum, CD4 + T percentage and CD4 + / CD8 + ratio plays an important role in the prognosis of HPV infection in different cervical lesions.