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目的建立保留全胃、不同区段小肠转流手术动物模型,探讨术后GK大鼠糖代谢的变化规律。方法 GK大鼠20只随机分为4组(n=5),Ⅰ组:假手术组;Ⅱ组:十二指肠转流组;Ⅲ组:空肠转流组;Ⅳ组:回肠转流组。分别于术前及术后4周行口服糖耐量试验及胰岛素耐量试验,计算胰岛素敏感指数(ISI)。结果 (1)全部GK大鼠术后均长期存活;(2)与Ⅰ组空腹血糖相比,各实验组术后空腹血糖明显下降;(3)与Ⅰ组血糖水平相比,各实验组在OGTT各时间点血糖水平明显下降;(4)与术前ISI相比,各实验组术后ISI增加,以Ⅲ组、Ⅳ组更为显著。结论成功建立保留全胃、不同区段小肠转流手术的GK大鼠模型;GBP效应区域是回肠;术后GK大鼠糖代谢改善与胰岛素敏感性增加有关,与胃容积无关。
Objective To establish an animal model of preserving small intestine bypass in the whole stomach and in different sections and to investigate the changes of glycometabolism in postoperative GK rats. Methods GK rats were randomly divided into 4 groups (n = 5), group Ⅰ: sham operation group, group Ⅱ: duodenal bypass group, group Ⅲ: jejunal bypass group, group Ⅳ: ileal commutation group . Oral glucose tolerance test and insulin tolerance test were performed preoperatively and 4 weeks after operation, and insulin sensitivity index (ISI) was calculated. Results (1) All GK rats survived after operation for a long time. (2) Compared with fasting blood glucose in group Ⅰ, the fasting blood glucose of each experimental group decreased significantly. (3) Compared with the blood glucose level in group Ⅰ, OGTT at each time point significantly decreased blood glucose levels; (4) Compared with the preoperative ISI, ISI increased in each experimental group, the group Ⅲ, Ⅳ more significant. Conclusion The GK rat model that preserves the whole stomach and different sections of small bowel bypass surgery was successfully established. The GBP effect region was ileum. The improvement of glucose metabolism in GK rats was related to the increase of insulin sensitivity, but not to the gastric volume.