一、目的 近年来表明有机体的各种环境因素,特别是正常菌丛,对于有机体的免疫反应能够产生各种影响,因此,往往使用无菌小鼠、无特殊病原菌(SPF)小鼠等进行研究。然而,如果使常规条件下育成的小鼠无菌化,就能多方探讨菌丛对免疫反应的影响。我们就是用口服非吸收性抗菌素的方法,使肠道正常菌丛无菌化后研究有机体的免疫功能, I. Purpose In recent years, various environmental factors of organisms, especially normal flora, have been shown to exert various effects on the immune response of organisms. Therefore, researches on sterile mice, mice without special pathogens (SPF) and the like are often conducted . However, if mice bred under normal conditions are sterilized, the effect of the bacterial flora on the immune response can be explored in many ways. We are using oral non-absorbent antibiotics to make the normal intestinal flora sterile after the study of immune function,