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目的探讨ABCG2基因沉默对人肺腺癌细胞GLC-82体内增殖的影响。方法将人肺腺癌细胞GLC-82、绿色增强荧光蛋白(EGFP)标记的细胞GLC-82/EGFP+与ABCG2基因沉默的细胞GLC-82/EGFP+/ABCG2-分别接种于裸鼠皮下,用免疫组化SP法及银染法,检测成瘤后瘤组织中细胞增殖指数K i-67的变化与核仁组成区嗜银蛋白(AgNOR)在瘤细胞中的变化,测试肿瘤细胞Ag-NOR颗粒截面数、单个AgNOR颗粒面积、单个核AgNOR总面积,并用图像分析系统对ABCG2蛋白在瘤细胞中的表达进行定量分析。结果裸鼠体内GLC-82/EGFP+/ABCG2-细胞成瘤组织与GLC-82、GLC-82/EGFP+细胞成瘤组织相比,前者ABCG2蛋白的表达明显减弱(P<0.05),K i-67增殖指数显著降低(P<0.05)且瘤细胞AgNORs颗粒截面数、单个核内AgNOR总面积明显减少,而单个AgNORs颗粒面积增大,差异有显著性(P<0.05)。结论沉默ABCG2基因能抑制人肺腺癌细胞GLC-82在体内的增殖。
Objective To investigate the effect of ABCG2 gene silencing on the proliferation of human lung adenocarcinoma cell line GLC-82. Methods Human GLC-82 / EGFP-labeled cells GLC-82 / EGFP + and GLC-82 / EGFP + / ABCG2- cells with ABCG2 gene silencing were inoculated subcutaneously in nude mice, SP method and silver staining were used to detect the change of K i-67 in the tumor tissue and the change of AgNOR in the tumor cells. The Ag-NOR particle cross section Number, single AgNOR particle area, single nucleus AgNOR total area, and quantitative analysis of ABCG2 protein expression in tumor cells by image analysis system. Results Compared with the tumorigenicity of GLC-82 and GLC-82 / EGFP + cells, the expression of ABCG2 protein in GLC-82 / EGFP / ABCG2 cells in nude mice significantly decreased (P <0.05) (P <0.05). The number of AgNORs particles in the tumor cells, the total area of AgNOR in single nucleus decreased significantly, while the area of single AgNORs particles increased, the difference was significant (P <0.05). Conclusion Silencing ABCG2 gene can inhibit the proliferation of human lung adenocarcinoma cell line GLC-82 in vivo.