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目的对加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠的病理和病变程度进行定量分析。方法采用人血清白蛋白(8 g/L)对大鼠进行皮下免疫,在大鼠产生抗体后分组[将造模大鼠随机分为6组,即加味鳖甲煎丸大(13g/kg)、中(6.5g/kg)、小(3.25g/kg)剂量组(简称为鳖甲大、中、小剂量组),模型组,阳性药秋水仙碱(1.0 mg/kg)对照组(简称秋水仙组),阳性药肝脾康(22.23 mg/kg)对照组(简称为肝脾康组)]给药,给药后40 min再由尾静脉注射人血清白蛋白,即可得到肝纤维化实验动物模型,实验结束后处死大鼠,病理切片,HE和Masson染色,对肝组织病理变化、胶原纤维含量进行分析。结果与模型组比较,鳖甲大、中、小剂量组能显著改善肝纤维化大鼠病变程度(总分降低率分别为62.50%、40.75%、8.33%);胶原纤维含量亦明显减少(P<0.05,P<0.01)。结论加味鳖甲煎丸对白蛋白所致免疫性肝纤维化大鼠病变程度有明显防治作用。
Objective To quantitatively analyze the pathological and pathological changes in rat liver fibrosis induced by albumin. Methods The rats were subcutaneously immunized with human serum albumin (8 g/L) and grouped after the rats produced antibodies [The model rats were randomly divided into 6 groups, namely, Jiawei Biejia Jian Wan (13 g/kg), Medium (6.5g/kg), small (3.25g/kg) dose group (abbreviated as Lijia large, medium, and small dose group), model group, positive drug colchicine (1.0 mg/kg) control group (abbreviated as autumn Narcissus group), positive drug liver spleen Kang (22.23 mg/kg) control group (abbreviated as liver and spleen Kang group) administration, 40 min after administration, then by intravenous injection of human serum albumin, you can get liver fibrosis Experimental animal model, after the end of the experiment, the rats were killed, pathological sections, HE and Masson staining, and the pathological changes of liver tissue and collagen fiber content were analyzed. Results Compared with the model group, the large, medium, and small doses of Radix Astragali significantly improved the pathological changes of liver fibrosis in rats (the total score reduction rates were 62.50%, 40.75%, and 8.33%, respectively); collagen fiber content also decreased significantly (P <0.05, P<0.01). [Conclusion] Jiawei Biejiajian Pill has obvious prevention and cure effect on albumin-induced immune fibrosis in rats.