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目的:研究胸腺素α原(Prothymosinα,ProTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yoelii-17XNL全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yoelii-17XNL全蛋白+ProTα;B组免疫P.yoelii-17XNL全蛋白;C组只注射ProTα;D组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yoelii-17XL,1×107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,B、D组小鼠全部死亡。结论:用P.yoelii-17XNL全蛋白做抗原,用ProTα作为佐剂,比单独用P.yoelii-17XNL全蛋白对小鼠有更好的免疫保护作用,提示了ProTα可以成为一种有潜力的蛋白疫苗。
Objective: To investigate the role of prothymosin α (ProTα) as immune adjuvant in Plasmodium yoelii vaccine. Methods: The whole protein of P.yoelii-17XNL was extracted as antigen, and the mice were immunized with thymosin α as an immunoadjuvant. The specific protocol was as follows: Kunming mice were divided into 4 groups with 6 mice in each group, group A immunized with P.yoelii-17XNL whole protein + ProTα; group B immunized with P.yoelii-17XNL whole protein; group C injected with ProTα only; Blank control, with the same volume of saline instead. Infection killed P. yoiiii-17XL, 1 x 107 worms / mouse after immunization. Results: In the first 10 days after infection, the average value of Plasmodium falciparum in group A was lower than the other three groups, and eventually 3 mice survived with a survival rate of 50%. One group of mice survived in group C, D group of mice all died. CONCLUSIONS: Using P.yoelii-17XNL whole protein as an antigen and ProTα as an adjuvant demonstrated better protection of mice than P.yoelii-17XNL whole protein alone, suggesting that ProTalpha may be a potential Protein vaccine.