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本文报告了人重组和杂交的α干扰素(IFN-α)对巨噬细胞介导的杀肿瘤活性有调节作用。人IFN-α-A/D可抑制小鼠IFN-γ激活小鼠腹腔巨噬细胞的杀肿瘤活性,而人IFN-α-A、-D和-A/D均可激活人单个核细胞的杀肿瘤活性。作者用小鼠腹腔巨噬细胞和健康人外周血单个核细胞作为效应细胞,分别用小鼠B16-F10肿瘤细胞和人黑素瘤A375细胞株作为靶细胞。效应细胞加IFN-γ和微量脂多糖(作激活效应细胞的第二信号)等处理18～24小时后,加入~(125)I UdR标记的靶细胞,效应
Here we report that human recombinant and hybridized interferon alpha (IFN-alpha) regulates macrophage-mediated tumoricidal activity. Human IFN-α-A/D inhibits the tumoricidal activity of mouse IFN-γ-activated mouse peritoneal macrophages, whereas human IFN-α-A, -D, and -A/D can activate human mononuclear cells. Kill tumor activity. The authors used mouse peritoneal macrophages and healthy human peripheral blood mononuclear cells as effector cells and used mouse B16-F10 tumor cells and human melanoma A375 cell lines as target cells, respectively. Effector cells plus IFN-γ and trace lipopolysaccharides (the second signal that activates effector cells) are treated for 18 to 24 hours, then ~(125)I UdR-labeled target cells are added.