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目的探讨三聚氰胺染毒对大鼠肾组织c-myc,c-fos和N-ras基因mRNA表达水平的影响。方法用三聚氰胺对SD大鼠进行亚急性经口染毒,设1个对照组和3个实验组,每天对动物采用经口灌胃染毒1次,染毒时间28d,试验结束时取大鼠肾组织进行荧光定量PCR检测c-myc,c-fos和N-ras基因mRNA表达水平。结果雄性大鼠三聚氰胺染毒后c-myc、c-fos、N-ras mRNA表达水平分别为1.72～3.39,2.34～4.03,2.92～12.08,与对照组比较明显增强(P<0.05,或P<0.01);雌性大鼠三聚氰胺染毒后c-myc、c-fos、N-ras mRNA表达水平分别为1.56～2.93,2.03～3.52,2.65～10.87,与对照组比较差异有统计学意义(P<0.05,或P<0.01)。结论三聚氰胺亚急性染毒引起大鼠肾组织部分癌基因表达水平升高。
Objective To investigate the effects of melamine exposure on mRNA expression of c-myc, c-fos and N-ras in rat kidney. Methods Subacute oral administration of melatonin to SD rats was used to establish a control group and three experimental groups. Animals were orally administered by oral gavage once daily for 28 days. Rats were sacrificed at the end of the experiment Renal tissues were examined by real-time PCR to detect the mRNA expression of c-myc, c-fos and N-ras. Results The mRNA expression levels of c-myc, c-fos and N-ras in melatonin of male rats were 1.72 ~ 3.39, 2.34 ~ 4.03 and 2.92 ~ 12.08 respectively, which were significantly increased compared with the control group (P < 0.01). The mRNA expression levels of c-myc, c-fos and N-ras in female rats after melamine exposure were 1.56-2.93, 2.03-3.52 and 2.65-10.87, respectively, which were significantly different from the control group (P < 0.05, or P <0.01). Conclusions Subacute exposure to melamine can cause the expression of some oncogenes in rat renal tissues.