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为了解海南岛正常人群风疹的免疫水平,1990年9月至1991年9月在海南岛中部和西部地区采集303人血清标本,应用 ELISA 法进行风疹 IgG 抗体检测,现将结果报告如下。一、材料与方法1.血清的来源:随机抽检不同年龄正常人303名,分离血清,-20℃保存待检。2.聚苯乙稀板系江苏泰县医疗器械厂产。酶联免疫检测仪系华东电子管厂产。病毒抗原与细胞抗原,酶结合物系中国预防医学科学院病毒学研究所提供。底物为邻苯二胺与 H_2(?)_2。3.检测方法及效价判定:血清风疹 IgG 抗体检测按《ELISA 检测风疹 IgG 抗体操作细则》。
In order to understand the immunization level of rubella in normal population of Hainan Island, 303 serum samples were collected in central and western Hainan Island from September 1990 to September 1991, and the rubella IgG antibody was detected by ELISA. The results are reported as follows. First, the material and methods 1. Source of serum: 303 randomly selected normal age, serum, -20 ℃ to be preserved. 2. Polystyrene board Jiangsu Taixian Medical Device Factory production. Enzyme-linked immunosorbent assay tube plant in east China. Viral antigens and cellular antigens, enzyme conjugates provided by the Chinese Academy of Preventive Medicine virology. The substrate for the o-phenylenediamine and H_2 (?) _ 2.3. Detection methods and determination of titer: serum rubella IgG antibody detection according to “ELISA test rubella IgG antibody handling rules.”