论文部分内容阅读
从我国云南省保山地区烟草上表现曲顶症状的植株上分离获得病毒分离物Y1, 经粉虱传毒及粒子形态观察, 证明为菜豆金色花叶病毒属(Begomovirus)病毒. 用14种单克隆抗体进行三抗体夹心ELISA测定, 结果表明Y1与我国及巴基斯坦、印度等国报道的双生病毒的抗原表位型均不同. 对Y1基因组DNA-A的全序列进行了分析, 全长2746个核苷酸, 其中病毒链含有两个ORF, 互补链含有4个ORF. Y1与其他双生病毒基因组DNA-A全序列、基因间隔区序列及各ORF编码的氨基酸序列的比较分析表明, Y1是一种新的Begomovirus病毒, 定名为烟草曲顶病毒, 英文名为Tobacco curly top virus, 简称TCTV. TCTV全基因组与印度报道的番茄曲叶病毒和番木瓜曲叶病毒的同源性最高, 达85%, 而其CP基因与巴基斯坦棉花曲叶病毒分离物72 b高度同源, 氨基酸水平的同源性达98%.
The isolate Y1 was isolated from the tobacco plants in Baoshan, Yunnan Province, China. The virus isolate Y1 was isolated from the plants infected with whitefly and the morphological observation of the particles showed that it was a Begomovirus. Antibody sandwich ELISA assay showed that Y1 and China and Pakistan, India and other countries reported twin antigen epitopes are different.Y1 genome DNA-A full-length sequence analysis, a total length of 2746 nucleosides Acid, in which the viral chain contains two ORFs and the complementary strand contains four ORFs. The comparative analysis of Y1 with the other genomic DNA-A sequences, the intergenic spacer sequences and the amino acid sequences encoded by the ORFs shows that Y1 is a novel Of the Begomovirus and named as Tobacco curly top virus (TCTV) .The whole genome of TCTV has the highest homology of 85% with the reported tomato leaf curl and papaya leaf curl in India The CP gene was highly homologous to the 72 b of cotton leaf curl isolate of Pakistan and the homology of amino acid was 98%.