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目的 观察后肢体缺血 /再灌注是否诱发心脏血红素氧合酶 1(HO - 1)的表达。方法 在氨基甲酸乙脂麻醉大鼠行后肢体缺血 /再灌注后 ,测量心脏组织中丙二醛的含量和超氧歧化酶 (SOD)的活性 ;并应用Northern和Western杂交方法分别检测HO - 1mRNA和蛋白的表达情况。结果 在肢体缺血 /再灌注过程中 ,心脏中的丙二醛含量增加 ,而超氧歧化酶的活性降低 ;单纯肢体缺血并不引起心脏血红素氧合酶 1mR NA和蛋白表达的变化 ,但缺血 /再灌注增强心脏血红素氧合酶 1mRNA和蛋白表达。结论 肢体缺血 /再灌注不仅提高心脏中丙二醛的含量 ,降低超氧歧化酶的活性 ;而且上调血红素氧合酶 1在心脏的表达。
Objective To observe whether hind limb ischemia / reperfusion induces the expression of heme oxygenase 1 (HO - 1). Methods The content of malondialdehyde (MDA) and superoxide dismutase (SOD) activity in heart tissue were measured after urethane anesthetized rats were subjected to limb ischemia / reperfusion. Northern blotting and Western blotting were used to detect the expression of HO - 1 mRNA and protein expression. Results In the process of limb ischemia / reperfusion, the content of malondialdehyde in the heart increased and the activity of superoxide dismutase decreased. Limb ischemia alone did not induce changes of cardiac hemoglobin (LOX) mRNA and protein expression, However, ischemia / reperfusion enhances cardiac heme oxygenase 1 mRNA and protein expression. Conclusion Limb ischemia / reperfusion can not only increase the content of malondialdehyde in the heart, decrease the activity of superoxide dismutase, but also increase the expression of heme oxygenase 1 in the heart.