论文部分内容阅读
以抗药性为标记,采用双标记菌株为亲本,研究了掘氏疫霉有性生殖行为。配对亲本 Phy607(A_1)、Phy64(A_2)抗放线菌酮对新霉素敏感,Phy115(A_1)、Phy112(A_2)抗新霉素对放线菌酮敏感。在配对组合 Phy607x Phy112、Phy115x Phy64 F_1代单卵孢株群体中,均出现同时抗放线菌酮和新霉素的个体,分别占群体的37%和50%.这种双重抗药性在单游动孢子无性系后代可稳定遗传,在自交后代发生分离。上述4个亲本在相对交配型产生的激素刺激下均可自交产生卵孢子。因此认为,掘氏疫霉种内菌株配对有性生殖可能以配对亲本杂交和各自自交等3种方式产生卵孢子,以双亲杂交为主要方式.
To drug resistance as a marker, the double marker strain as the parent to study the genitourinary behavior of Phytophthora dysenteriae. Paired parent Phy607 (A_1) and Phy64 (A_2) were resistant to neomycin. Phy115 (A_1) and Phy112 (A_2) were resistant to cycloheximide. In the pairing combination Phy607x Phy112, Phy115x Phy64 F_1 single-spawn population, both anti-cycloheximide and neomycin appeared in the population, accounting for 37% and 50% of the population, respectively. The progeny of the zoospore clones can be stably inherited and segregated in the selfed offspring. The above 4 parents can self-produce oospores under the stimulation of hormone produced by mating type. Therefore, it is suggested that P. diphtheriae strain mating sexual reproduction may produce oospores by 3 ways of matching parental cross and their own self-cross, with the parental cross as the main way.