【摘 要】
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Objective:To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STDP) following sodium laurate-induced coronary microembolization (CME) in rats.Methods:Forty rats were divided int04 groups:the control (sham) group,CME group,low-do
【机 构】
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Central Laboratory,Hangzhou First People’s Hospital,Zhejiang University School of Medicine,Hangzhou
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Objective:To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STDP) following sodium laurate-induced coronary microembolization (CME) in rats.Methods:Forty rats were divided int04 groups:the control (sham) group,CME group,low-dose STDP pretreatment group (20 mg·kg-1·d-1),and high-dose STDP pretreatment group (40 mg·kg-1·d-1).The rats were intragastric administrated with STDP 2 weeks before operation.Moreover,the histopathological alterations were observed using optical microscopy and transmission electron microscopy.Antioxidant biomarkers were analyzed by enzyme-linked immunosorbent assay.Mitochondrial functions including the mitochondrial permeability transition pore (mPTP) mtDNA copy number were determined and proteins of AKT/GSK3β were analyzed by Western blot.Results:The rats in the CME group showed a significant increase in the fibrinogen-Iike protein 2 expression level and mitochondrial dysfunction and a decrease in the expression level of antioxidant biomarkers (superoxide dismutase and catalase,P＜0.01 for all).In contrast,the rats in the low-and high-dose STDP pretreatment groups showed a significant decrease in coronary microthrombi (P＜0.05);moreover,STDP restored the antioxidant-related protein activities and mitochondrial function,inhibited mPTP opening,decreased AKT-Ser473 phosphorylation,and increased GSK3β-Ser9 phosphorylation (P＜0.05 or P＜0.01).Conclusion:STDP may be useful for treatment of CME,possibly via regulation of mPTP opening and AKr/GSK3β phosphorylation.
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