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研究了水环境急性盐度胁迫对卵形鲳鲹(Trachinotus ovatus)幼鱼鳃Na+-K+-ATP(NKA)酶活力,血清、鳃丝和肾脏渗透压的影响.结果表明:将幼鱼从盐度30(对照)中直接转移至盐度5、10、15、20、25、35水体中,96 h后无死亡.各盐度处理组的鳃NKA酶活性和血清渗透压在最初72 h内出现一定波动,随后变化平稳.试验结束时(96 h),NKA活性随盐度梯度呈“U”型分布,盐度35处理的酶活高于其他处理,盐度20处理活性最低.各处理的血清渗透压大小在96 h时,随着盐度的变化,以盐度15、20为中心,呈对称变化,在盐度20后随盐度上升呈先上升后下降的趋势.相同盐度的鳃渗透压随时间变化呈先上升后下降逐渐稳定的趋势.肾渗透压除盐度5、10处理外,其他盐度组随时间没有显著变化,维持一定的稳定性.说明卵形鲳鲹幼鱼在生理上具有广盐性鱼类的“高渗环境高NKA活性”特征,有较强的渗透压调节与平衡能力.
The effects of acute salinity stress on the Na + -K + -ATP (NKA) activity, serum, gill and kidney osmotic pressure in the gill of Trachinotus ovatus were studied. The results showed that: Degree 30 (control) to salinity 5, 10, 15, 20, 25, 35. No death after 96 h.The gill NKA activity and serum osmolality in each salinity treatment group were within the first 72 h (96 h), the activity of NKA was distributed as “U” with the salinity gradient. The activity of salinity 35 was higher than that of other treatments, and the salinity 20 treatment activity was the lowest. With the increase of salinity, the osmotic pressure of each treatment showed a symmetrical change with the salinity of 15 and 20 at 96 h, and then increased with the increase of salinity at salinity 20 and then decreased at the same time Salinity gill osmotic pressure showed a trend of first rising and then falling gradually stable .In addition to the salinity 5,10 treatment, the other salinity groups did not change significantly over time, maintaining a certain degree of stability. Shad juveniles physiologically have broad-salt fish “hypertonic environment of high NKA activity ” features a strong osmoregulation and balance of energy .