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Objective: We aimed to provide an alternative cell source for cell therapy in leukoaraiosis(LA). Methods: Olfactory ensheathing cells(OECs) from the olfactory bulb were isolated,cultured, and purified. Next, the lentivirus carrying human VEGF165 gene was constructed and transfected into OECs. Results: The proliferative capacity of primary OECs was strong. OECs were infected with different multiplicity of infection. The expression level of VEGF was confirmed by real-time PCR with specific primers for GAPDH and VEGF, indicating that the genetically engineered OECs-VEGF produced VEGF with functional activity. Conclusions: Our data showed that these engineered OECs-VEGF highly express functional VEGF and retain the characteristics of astrocytes and Schwann cells,providing an alternative cell source for cell therapy in LA.
Objective: We aimed to provide an alternative cell source for cell therapy in leukoaraiosis (LA). Methods: Olfactory ensheathing cells (OECs) from the olfactory bulb were isolated, cultured, and purified. Next, the lentivirus carrying human VEGF165 gene was constructed and The expression level of VEGF was confirmed by real-time PCR with specific primers for GAPDH and VEGF, indicating that the genetically engineered OECs -VEGF produced VEGF with functional activity. Conclusions: Our data showed those engineered OECs-VEGF highly express functional VEGF and retain the characteristics of astrocytes and Schwann cells, providing an alternative cell source for cell therapy in LA.