论文部分内容阅读
根据已发表的长角血蜱16SrRNA序列及斑点热群立克次体外膜蛋白A(OmpA)基因序列设计2对特异性引物,对唐山地区采集的长角血蜱进行PCR检测,并对阳性样本进行测序和序列分析,抽检样本建立分子系统进化树。结果表明,在315份蜱DNA样本中检测出25份阳性样本,阳性率为7.94%;序列分析结果显示唐山地区长角血蜱携带立克次体同处于一个分支,与日本株立克次体同源性最高(93.30%),其次是福建株立克次体(92.11%),黑龙江立克次体绥芬株(90.45%)、虎林株(90.42%)。结论得出唐山地区蜱传斑点热感染较严重,分子进化分析结果显示唐山地区蜱传斑点热群立克次体可能为一新种。
Two pairs of specific primers were designed according to published sequences of 16S rRNA and OmpA gene sequence of C. longicornis, PCR was carried out on the collected H. pylori from Tangshan area, and the positive samples Sequencing and sequence analysis were performed and samples were taken to establish a molecular phylogenetic tree. The results showed that in 315 tick tick DNA samples, 25 positive samples were detected, the positive rate was 7.94%. The sequence analysis showed that T. rhamnosus carried T. rhamnosus in one branch with T. rickettsii The highest homology was 93.30%, followed by Fujian Rickettsia (92.11%), Heilongjiang Rickettsia Suifen (90.45%) and Hulin (90.42%). The conclusion is that Tangshan area is more likely to have tick-borne thermo-infectives, and the molecular evolutionary analysis shows that tick-spotted spot-hot clots Rickettsia may be a new species in Tangshan area.