通道蛋白家族NIPs(NOD26-like intrinsic proteins)是植物中重要的硼吸收与转运蛋白。利用同源克隆技术获得“金魁”猕猴桃(Actinidia deliciosa‘jinkui’)硼转运通道基因:AdNIP5-1,该基因序列包括897 bp的开放阅读框,编码298个氨基酸。AdNIP5-1蛋白包含通道蛋白家族2个典型的保守结构域:NPS、NPV和Ar/R,构建基因家族进化树分析AdNIP5-1属于NIPⅡ亚家族,与葡萄和拟南芥中的NIP 5-1蛋白序列比对一致性分别为:89%和82%。利用实时荧光定量分析表明AdNIP5-1主要在猕猴桃根部表达,在茎和叶中的表达量相对较低;在缺硼胁迫条件下,AdNIP5-1表达量显著升高,在12 h时达到最高值,为对照的2.1倍。结果表明AdNIP5-1基因参与缺硼胁迫响应过程,该基因可能会影响猕猴桃对硼的吸收效率。 NOD26-like intrinsic proteins are important boron uptake and transport proteins in plants. The homologue cloning technique was used to obtain the gene encoding AdNIP5-1, an open reading frame of 897 bp, encoding 298 amino acids of Actinidia deliciosa ’jinkui’. The AdNIP5-1 protein contains two typical conserved domains of the channel protein family: NPS, NPV and Ar / R. Construction of Gene Family Phylogenetic tree analysis AdNIP5-1 belongs to the NIPII subfamily and is associated with NIP 5-1 in grapes and Arabidopsis The alignments of protein sequences were 89% and 82%, respectively. Quantitative real-time fluorescence analysis showed that AdNIP5-1 was mainly expressed in the root of kiwifruit and relatively low in stem and leaf. Under the condition of boron deficiency, the expression of AdNIP5-1 was significantly increased and reached its peak at 12 h , 2.1 times of the control. The results showed that AdNIP5-1 gene involved in boron deficiency stress response process, the gene may affect Kiwi fruit boron absorption efficiency.