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采用滴定法制备紫苏子油纳米乳,用激光粒度测定仪分析其粒径,并进行其对体外培养的小鼠乳腺癌细胞EMT-6的凋亡诱导试验。结果显示,制备的紫苏子油纳米乳外观澄明,平均粒径为(50.3±1.1)nm。与紫苏子油软胶囊相比,质量浓度为100~800 mg/L时的紫苏子油纳米乳较紫苏子油软胶囊对EMT-6细胞的诱导凋亡作用均强,差异显著(P<0.05)。透射电镜下,EMT-6细胞染色质边集,胞质内出现空泡,形成凋亡小体。800 mg/L剂量紫苏子油纳米乳处理EMT-6细胞48 h后,凝胶电泳可见明显的“阶梯状”DNA条带,为180~200 bp整数倍递增分布,表明紫苏子油纳米乳具有明显的诱导EMT-6细胞凋亡的作用。
Perilla seed oil nanoemulsion was prepared by titration method, its particle size was analyzed by laser particle size analyzer, and its apoptosis induction test on mouse breast cancer cell EMT-6 cultured in vitro was carried out. The results showed that the appearance of the perilla seed oil nanoemulsion was clear and the average particle size was (50.3 ± 1.1) nm. Compared with Perilla frutescens oil soft capsule, the perilla seed oil perilla seed oil had stronger effect of inducing apoptosis on the EMT-6 cells when the mass concentration was 100-800 mg / L P <0.05). Under the transmission electron microscope, the chromatin margins of EMT-6 cells formed vacuolation in the cytoplasm to form apoptotic bodies. After EMT-6 cells were treated with 800 mg / L perilla seed oil for 45 h, the obvious “ladder-like” DNA bands were observed in gel electrophoresis, which was an integral multiple of 180-200 bp, indicating that Perilla seed Oil nanoemulsion has obvious effect of inducing apoptosis of EMT-6 cells.