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目的：对３８例人肝细胞癌（ＨＣＣ）及癌旁组织、５例正常肝组织作层粘连蛋白（ＬＮ）及其受体（６７ＬＲ）和Ⅳ型胶原（ＣｏｌⅣ）定位和定量研究。方法：应用免疫组织化学及图像分析。结果：高分化组ＬＮ和ＣｏｌⅣ围绕梁索或腺样结构呈连续线型分布，中、低分化组呈段、点状，ＨＣＣ浸润前缘两者残缺。６７ＬＲ主要于癌细胞膜表面和／或胞浆内表达，其表达水平明显高于癌旁及正常肝组织，尤以低分化组为突出。１８／３８例ＨＣＣ伴肝内浸润和／或转移者，其原发灶内６７ＬＲ表达明显高于无肝内浸润和转移组（Ｐ值＜０．０１）。静脉内癌栓瘤细胞更多表达胞膜型６７ＬＲ。结论：基膜蛋白及ＬＮ受体表达变化与ＨＣＣ浸润、转移和细胞分化程度相关。
OBJECTIVE: To study the localization and quantitative study of laminin (LN), its receptor (67LR) and type IV collagen (ColIV) in 38 cases of human hepatocellular carcinoma (HCC) and adjacent tissues and 5 cases of normal liver tissues. Methods: Application of immunohistochemistry and image analysis. RESULTS: In the well-differentiated group, LN and Col IV showed a continuous linear distribution around the girdle or adenoid structure. The middle and poorly differentiated groups presented segments and points. The invasive front of HCC was incomplete. 67LR is mainly expressed on the surface and/or cytoplasm of cancer cell membranes. The expression level of 67LR is significantly higher than that of paracancerous and normal liver tissues, especially in the poorly differentiated group. Among 18/38 cases of HCC with intrahepatic infiltration and/or metastasis, the expression of 67LR in the primary tumor was significantly higher than that in the group without liver infiltration and metastasis (P<0.01). Intravenous tumor-bearing tumor cells express more of the membrane type 67LR. CONCLUSIONS: The changes in the expression of basement membrane proteins and LN receptors are related to the extent of HCC infiltration, metastasis, and cell differentiation.