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【目的】了解新疆春小麦材料脂肪氧化酶(LOX)活性基因、黄色素含量基因Ta Zd-A1与Ta Zd-D1的等位变异组成和分布特点,分析Ta Zd-A1、Ta Zd-D1与Ta LOX-B1基因分子标记的实用性,为新疆春小麦品质改良提供参考依据。【方法】利用LOX16、LOX18、YP2A-1、YP2D-1特异性分子标记,对167份春小麦材料进行Ta Lox-B1a、Ta Lox-B1b、Ta Zds-A1a、Ta Zds-A1b、Ta Zds-D1a和Ta Zds-D1b等位基因的分子检测,明确这类基因在新疆春小麦材料中的分布规律。【结果】表明在检测的材料中控制籽粒黄色素含量的两个主效位点Ta Zds-A1和Ta Zds-D1存在4种不同的等位变异组合类型,以Ta Zds-A1b/Ta Zds-D1a组合基因所占比例最多,为29.34%;含有低黄色素含量Ta Zds-A1a/Ta Zds-D1b基因组合的材料为21.56%;同时,不同来源的春小麦材料所含等位变异组合类型不尽相同,中亚国家材料以Ta Zds-A1a/Ta Zds-D1b最多,为48.0%;新疆选育的品种中含Ta Zds-A1a/Ta Zds-D1b组合类型的材料只有5.4%,可见低黄色素的Ta Zds-A1a/Ta Zds-D1b基因类型在新疆春小麦材料中相对匮乏。在167份材料中,扩增出Ta LOX-B1a的有66份,属于高LOX活性的等位基因型,为39.52%。而新疆选育品种出现Ta Lox-B1a基因的频率远低于CIMMYT材料和中亚国家材料。【结论】利用的分子标记检测结果稳定可靠,筛选出一些低YP含量与高LOX活性的材料,可作为选育品种的亲本,以加速小麦色泽品质育种改良的效率。
【Objective】 The objective of this study was to understand the allelic variation of TaZd-A1 and Ta Zd-D1 gene and the distribution of TaZd-A1, Ta Zd-D1 and Ta Zd-D1 genes in Xinjiang wheat. The practicality of LOX-B1 gene molecular marker provides a reference for the quality improvement of spring wheat in Xinjiang. 【Method】 The expression of Ta Lds-B1a, Ta Lox-B1b, Ta Zds-A1a, Ta Zds-A1b and Ta Zds-D1a in 167 spring wheat cultivars were detected by LOX16, LOX18, YP2A-1 and YP2D- And Ta Zds-D1b allele molecular detection, clear the distribution of these genes in Xinjiang spring wheat material. 【Result】 The results showed that there are 4 different combinations of allelic variation in Ta Zds-A1 and Ta Zds-D1 controlling grain yellow pigment content in the tested materials. Ta Zds-A1b / Ta Zds- D1a gene accounted for the highest proportion (29.34%). The genotypes containing TaZds-A1a / Ta Zds-D1b with low yellow pigment content were 21.56%. In addition, the types of allelic combinations of spring wheat from different sources were not enough In the same way, the materials of Ta Zds-A1a / Ta Zds-D1b in Central Asia are the most, which is 48.0%. The combinations of Ta Zds-A1a / Ta Zds-D1b in Xinjiang are only 5.4% The Ta Zds-A1a / Ta Zds-D1b genotype is relatively deficient in Xinjiang spring wheat. Among 167 materials, 66 strains of Ta LOX-B1a were amplified, belonging to the allele with high LOX activity, which was 39.52%. However, the frequencies of Ta Lox-B1a gene in breeding breeds of Xinjiang were much lower than those of CIMMYT and Central Asian countries. 【Conclusion】 The results of molecular marker detection were stable and reliable. Some materials with low YP content and high LOX activity were screened, which could be used as parents of breeding varieties to accelerate the improvement of wheat color quality breeding.