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目的由于转基因大豆及其制品日益增多,其安全性亦受到了越来越多消费者的关注。为满足消费者的知情选择权,本研究建立一种快速检测外源基因的方法。方法以转基因豆制品中存在的CaMV35S启动子和NOS终止子为检测目标,优化了二重PCR反应体系和条件,包括引物比例和酶的用量;运用聚丙烯酰胺凝胶电泳和琼脂糖凝胶电泳对PCR产物进行检测,并对聚丙烯凝胶电泳的银染条件进行探索研究。结果建立了运用聚丙烯酰胺凝胶电泳更好地区分出外源基因CaMV35S启动子和NOS终止子的目的条带的方法。结论本实验中的二重PCR和聚丙烯酰胺凝胶电泳方法适用于对转基因豆制品中这两个外源基因的检测,为转基因豆制品检测提供了指导。
Purpose Due to the growing number of genetically modified soybeans and their products, their safety has also attracted more and more consumers’ attention. In order to satisfy consumer’s informed choice, this study established a rapid detection of foreign genes. Methods The double-stranded PCR reaction system and conditions, including the ratio of primers and the amount of enzyme, were optimized with the presence of CaMV35S promoter and NOS terminator in transgenic soybean products. Polyacrylamide gel electrophoresis and agarose gel electrophoresis The PCR products were detected, and the silver staining conditions of polypropylene gel electrophoresis were explored. Results The method of using polyacrylamide gel electrophoresis to distinguish the target gene of exogenous gene CaMV35S promoter and NOS terminator was established. Conclusion The multiplex PCR and polyacrylamide gel electrophoresis in this experiment are suitable for the detection of the two exogenous genes in transgenic soybean products and provide guidance for the detection of genetically modified soy products.