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[目的]检测肌成纤维细胞标志物α-SMA和胶原纤维在人口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的表达情况,探讨它们的表达与癌侵袭方式等级积分(Invasive pattern grading score,IPGS)间的关系及对预后的影响。[方法 ]采用HE染色判定侵袭方式等级积分;Masson染色和免疫组化分析胶原面积百分比(collagen area percentage,CAP)和α-SMA的阳性百分率,并进行统计学分析。[结果]80例OSCC样本在IPGS<5组和≥5组的病例数分别为32和48例。α-SMA强阳性比例和CAP在IPGS<5组和≥5组存在差异,分别为P=0.004,P=0.001;Spearman分析验证了α-SMA与CAP呈负相关(r=-0.391)。IPGS<5患者生存时间长于IPGS≥5患者(P=0.001)。[结论]α-SMA在IPGS≥5的OSCC患者中的表达显著强于IPGS<5患者,胶原纤维表达与肌成纤维细胞的出现呈负相关。在OSCC中IPGS联合α-SMA和胶原纤维的表达可以作为OSCC患者的临床预后判断和诊断检测有价值的指标。
[Objective] To investigate the expression of α-SMA and collagen fibers in human oral squamous cell carcinoma (OSCC) and to explore the relationship between their expression and the invasiveness of cancer invasion (Invasive pattern grading score , IPGS) and the impact on prognosis. [Method] The invasion score was determined by HE staining. The positive percentage of collagen area percentage (CAP) and α-SMA was analyzed by Masson staining and immunohistochemistry, and the statistical analysis was made. [Results] The number of cases of OSCC in 80 cases with IPGS <5 and ≥ 5 were 32 and 48 respectively. The positive ratio of α-SMA and CAP were different between IPGS <5 and ≥5 groups, P = 0.004 and P = 0.001 respectively. Spearman analysis showed that α-SMA was negatively correlated with CAP (r = -0.391). Patients with IPGS <5 survived longer than those with IPGS> 5 (P = 0.001). [Conclusion] The expression of α-SMA in OSCC patients with IPGS≥5 is significantly stronger than those with IPGS <5. The expression of collagen fibers is negatively correlated with the appearance of myofibroblasts. The expression of IPGS combined with α-SMA and collagen fibers in OSCC can be used as a valuable index in the clinical prognosis and diagnosis of OSCC.