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目的:探讨逆行射精患者尿液中回收精子的冷冻保存及其在宫腔内人工授精(IUI)周期中的应用效果。方法:2008年12月至2011年1月逆行射精患者共计7例,嘱其按要求留取射精后的尿液,充分洗涤后实施液氮蒸汽法超低温精子冷冻,共冻存精液标本14人份。IUI周期时将冻存精子解冻、优化后行宫腔内人工授精。结果:冷冻前、解冻后前向运动精子总数分别为(19.9±10.4)×106和(6.9±4.2)×106,存在显著性差异(P<0.05),解冻优化后前向运动精子总数为(2.6±1.7)×106,实施IUI 8个周期,临床妊娠1例。结论:超低温冷冻会明显降低前向运动精子总数,但多次冷冻后一次复苏行IUI可能是治疗逆行射精所致不育的一种较为理想的方法。
Objective: To investigate the cryopreservation of urine collected from retrograde ejaculation patients and its application in intrauterine insemination (IUI) cycle. Methods: From December 2008 to January 2011, a total of 7 cases of retrograde ejaculation patients were asked to take urine after ejaculation as required. After washing sufficiently, cryopreservation of cryopreserved sperm by liquid nitrogen vapor method was performed. A total of 14 samples of frozen semen . Frozen sperm were thawed during IUI cycle, and intrauterine insemination was optimized. Results: The total number of forward motile sperm after thawing was (19.9 ± 10.4) × 106 and (6.9 ± 4.2) × 106, respectively, after thawing (P <0.05), and the total number of forward motile sperm after thawing was ( 2.6 ± 1.7) × 106, the implementation of IUI 8 cycles, 1 case of clinical pregnancy. CONCLUSIONS: Cryopreservation can significantly reduce the total number of forward motile spermatozoa. However, IUI may be an ideal treatment for infertility caused by retrograde ejaculation.