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应用聚酯纤维在培养基上形成的立体网架,接种生后7天大鼠视神经,然后对少突胶质细胞在三维空间的迁移进行体外培养观察。以抗髓鞘碱性蛋白抗体标记少突胶质细胞,并用扫描电镜和透射电镜对在聚酯纤维上迁移的少突胶质细胞超微结构进行了研究。结果表明,聚酯纤维能引导少突胶质细胞迁移;在其表面,少突胶质细胞的突起相互交错并将其包绕;有些突起已在其表面融合,形成“类膜”结构,有些部位可见有2～3层这样的膜性结构。免疫细胞化学染色结果表明,少突胶质细胞突起在聚酯纤维表面形成的“类膜”结构为髓鞘碱性蛋白阳性。以上结果提示,在体外培养条件下,聚酯纤维能引导少突胶质细胞迁移,在无神经元存在时,少突胶质细胞仍可形成髓鞘碱性蛋白阳性的膜性结构。本文对中枢神经系统髓鞘形成模式进行了讨论。
Using the three-dimensional network formed by polyester fiber on the medium, the optic nerve of rats was inoculated 7 days after birth, and then the migration of oligodendrocytes in three-dimensional space was observed in vitro. Oligodendrocyte cells were labeled with anti-myelin basic protein antibody, and the ultrastructure of oligodendrocytes migrating on polyester fibers were studied by scanning electron microscopy and transmission electron microscopy. The results show that polyester fibers can guide the migration of oligodendrocytes; on their surface, the protrusions of oligodendrocytes are interlaced and wrap around; some of the protrusions have fused on their surface to form a “membrane-like” structure, some There are 2 or 3 layers of membranous structure. Immunocytochemical staining results showed that the “membrane-like” structure of oligodendrocyte protuberances formed on the surface of polyester fibers was positive for myelin basic protein. These results suggest that in vitro culture conditions, polyester fibers can guide the migration of oligodendrocytes, in the absence of neurons, oligodendrocytes still can form myelin basic protein-positive membrane structure. This article discusses the central nervous system myelination patterns are discussed.