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用４ｍｍｏｌ／Ｌ浓度的赖氨酸加苏氨酸作为选择压，经相同浓度液体和固体选择培养基连续选择，从未经诱变处理的芹菜胚性愈伤组织筛选出一个稳定的赖氨酸加苏氨酸抗性细胞系并得到再生植株。抗性细胞系再生植株整株的氨基酸含量比对照组植株高１０倍左右。但是地上部分氨基酸含量，抗性细胞系再生植株比对照植株有所降低。两种植株叶片的细胞色素氧化酶同工酶谱有明显差异，抗性细胞系再生植株有４条谱带，而对照植株只有两条谱带，不同同工酶的相对酶活性也有所不同。本文还讨论了氨基酸抗性突变体选择中，有关氨基酸合成和积累方面一些值得注意的问题。
With 4 mmol / L lysine and threonine as the selection pressure, the medium was selected by the same concentration of liquid and solid selection medium, and a stable lysine was screened from the embryogenic callus of celery without mutagenesis Plus threonine resistant cell lines and regenerated plants. Resistant cell lines regenerated plants whole plant amino acid content than the control plant about 10 times higher. However, the aerial parts of amino acid content, resistant cell lines regenerated plants decreased compared with the control plants. The cytochrome oxidase isoenzymes of two plant leaves were significantly different, the resistant cell line regenerated plants have 4 bands, while the control plants only two bands, different isozymes relative enzyme activity is also different. This article also discusses some notable issues in the selection of amino acid resistant mutants for amino acid synthesis and accumulation.