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目的 探讨辐射诱导启动子调控的造血因子基因表达对照射后造血保护的作用。方法 将构建的携带有Egr 1启动子的Flt3配基 (FL)cDNA和增强型绿色荧光蛋白 (EGFP)cDNA双顺反子表达载体导入基质细胞后 ,联合人CD34 +细胞输入经亚致死剂量照射的重症联合免疫缺陷(SCID)小鼠体内 ,观察外周血象动态改变和人造血细胞及其基质细胞植入的变化。结果 实验组与对照组相比外周血白细胞下降幅度明显减轻 ,恢复加快 ,骨髓可见绿色荧光阳性的基质细胞 ,而各组间骨髓中CD45 +细胞、CD34 +细胞、CFU GM及骨髓有核细胞计数差异无显著性。结论 Egr 1启动子调控的造血因子基因疗法具有一定的辐射造血保护作用
Objective To investigate the effect of hematopoietic gene expression regulated by radiation-induced promoter on hematopoietic protection after irradiation. METHODS: Flt3 ligand (FL) cDNA and EGFP cDNA bicistronic expression vector carrying Egr1 promoter were constructed and transfected into stromal cells. The combined CD34 + cells were exposed to sublethal radiation Of severe combined immunodeficiency (SCID) mice to observe the dynamic changes of peripheral blood and the changes of human hematopoietic cells and their stromal cells. Results Compared with the control group, the decrease of peripheral blood leukocytes in experimental group and control group was faster than that in control group. The green fluorescent positive stromal cells were found in bone marrow, while the numbers of CD45 + cells, CD34 + cells, CFU GM and bone marrow nucleated cells No significant difference. Conclusion The gene therapy of hematopoietic cells regulated by Egr 1 promoter has certain hematopoietic protective effects