【摘 要】
:
Current serum neutralization assays based on the inhibition of the eytopathic effect(Nt-CPE) need to manipulate live viruses,which are time-consuming,labor-inte
【机 构】
:
National Engineering Laboratory for AIDS Vaccine,Department of Pediatrics,Changchun Baike Biotechnol
【基金项目】
:
Supported by the National Natural Science Foundation of China
论文部分内容阅读
Current serum neutralization assays based on the inhibition of the eytopathic effect(Nt-CPE) need to manipulate live viruses,which are time-consuming,labor-intensive,and have the potential exposure to infectious agents,so a safe and objective assay via pseudovirus for the fast and efficient detection of enterovirus 71(EV71 ) neutralizing antibodies was developed.First,we generated EV71 pseudovirus containing firefly luciferase gene in place of the capsid gene P1 in EV71 genome.Vero cells infected with 200 CCID50(50% cell culture infective dose) of EV71 pseudovirus for 24 h were found to have the best performance.Seval sera were measured by EV71 pseudoparticle neutralization assay(Nt-PPN) and the conventional serological method Nt-CPE.Neutralizing antibody titers measured by Nt-PPN and those obtained by Nt-CPE demonstrate a high correlation between the two methods.Overall,the PPN assay represents a valid alternative to conventional serological methods for the evaluation of EV71 neutralizing antibodies.This method can be used for detecting neutralizing antibodies of other picornaviruses,such as hepatitis A virus(HAV) and coxsackievirus 16(CVA16),and make it possible to determine whether there is cross-reactivity between EV71 and CVA16.
其他文献
spindlinl为作者所在研究组克隆并命名的肿瘤相关新基因,之前研究表明spindlin1蛋白定位丁细胞核,并有可能通过对TCF-4通路的调节参与对肿瘤细胞生长和周期的调控.为进一步探
MBE growth of ZnSxSe1-x thin films on ITO coated glass substrates were carried out using ZnS and Se sources with the substrate temperature ranging from 270℃ to
本文介绍了现代烟草农业的相关概念和基本特征,讨论了现代烟草农业的品牌建设,最后以安康山地烤烟品牌建设为例,探讨了烟叶品牌建设的途径。
For the pre-acquired serial images from camera lengthways motion, a view synthesis algorithm based on epipolar geometry constraint is proposed in this paper. It
By choosing different weight contents of Ni and Cu,Mo alloys have been densified under 1473K-30MPa-1h in a vacuum furnace.The experimental results indicate that
机械化秸秆还田技术有效地改善了土壤有机质,提高土壤供肥能力,增加粮食产量,让农民朋友充分了解秸秆综合利用技术的科学性、实用性、经济性和社会性。从而有效地杜绝秸秆焚烧带
随着“三北”四期防沙、治沙、退耕还林、村屯绿化造林工程的发展,我县杨树人工林面积不断增多。杨树溃疡病以及钻蛀性病害对杨树成林和幼树的危害越来越重,严重发生地病害率达
石楠 (国际城市与区域规划师学会副主席,中国城市规划学会副理事长兼秘书长,本论坛主持人):非常欢迎大家参加这个关于教育和人才的论坛.参加组织这个讨论的有四家机构的代表,
6-(Bromomethyl)-4-methoxy-5,6,7,8-tetrahydropyrido[3,2-d]pyrimidin-2-ol(compound 9) was synthesized from 6-methylpyrimidine-2,4-diol(compound 1) through the cru
首先提出以音素合并后模型自身似然度下降为距离依据,通过聚类生成多语言通用音素的声学建模方法.在此基础上,比较聚类时增加两种约束条件(同一语种内音素不聚类、不同IPA族