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目的研究不育症患者精核蛋白mRNA表达量的变化及意义。方法提取6例不育症患者及5例健康男性精子的总RNA,应用RT-PCR法扩增得到精核蛋白cDNA片段;提取精子总碱性核蛋白并进行SDS-聚丙酰胺凝胶电泳,检测其中精核蛋白及组蛋白的含量。结果精子中存在精核蛋白基因的mRNA;不育症患者精核蛋白的含量及精核蛋白mRNA相对含量均下降。结论精核蛋白mRNA相对含量的变化与其蛋白含量的变化一致,可能反映了精子发生过程中某些基因的表达情况。
Objective To study the changes and significance of the expression of protamine mRNA in infertile patients. Methods Total RNA was extracted from 6 infertile patients and 5 healthy male sperm. The cDNA fragments of sperm nucleus were amplified by RT-PCR. Sperm total alkaline nucleoprotein was extracted and analyzed by SDS-polyacrylamide gel electrophoresis. Which protien protein and histone content. Results There were mRNA of sperm nucleoprotein gene in sperm. The content of sperm nucleus protein and relative content of sperm protein mRNA in infertile patients decreased. Conclusion The changes of the relative content of mRNA of sperm nucleus are consistent with the changes of its protein content, which may reflect the expression of certain genes during spermatogenesis.