论文部分内容阅读
目的:研究神经酸对帕金森病(PD)模型小鼠运动障碍的缓解作用及机制。方法:将小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、多巴丝肼片组(阳性对照,按左旋多巴计120 mg/kg)和神经酸低、中、高剂量组(20.0、40.0、80.0 mg/kg),每组10只。除空白对照组外,其余各组小鼠均建立PD模型。成模后,每天ig相应药物1次,连续14 d。末次给药后,观察各组小鼠行为学变化,采用高效液相色谱法测定小鼠脑纹状体内多巴胺(DA)及其代谢物二羟苯乙酸(DOPAC)、高香草酸(HVA)的含量。结果:与空白对照组比较,模型组小鼠爬杆时间延长、滚筒时间缩短、自发运动次数减少(P<0.05),脑纹状体内DA、DOPAC、HVA含量均降低(P<0.05)。与模型组比较,多巴丝肼片组和神经酸各剂量组小鼠爬杆时间缩短、滚筒时间延长(P<0.05),脑纹状体内DA、DOPAC、HVA含量均升高(P<0.05);多巴丝肼片组和神经酸高剂量组小鼠自发运动次数增加(P<0.05)。结论:神经酸可有效改善PD模型小鼠的运动障碍症状,其机制可能与增加脑纹状体内DA含量有关。
Objective: To investigate the neuroprotective effects and mechanism of Nerve acid on dyskinesia in mice with Parkinson’s disease (PD). Methods: The mice were randomly divided into blank control group (saline), model group (normal saline), dopprous hydrazine tablets group (positive control, 120 mg / kg levodopa) High-dose group (20.0,40.0,80.0 mg / kg), 10 in each group. Except the blank control group, PD mice were established in all other groups. Into the mold, the corresponding drug 1 ig daily for 14 days. After the last administration, the behavioral changes of mice in each group were observed. The content of dopamine (DA) and its metabolites, DOPAC, HVA, in the striatum of mice were measured by high performance liquid chromatography content. Results: Compared with the blank control group, the rats in the model group had longer climbing time, shorter roller time and fewer spontaneous movement events (P <0.05), and decreased the content of DA, DOPAC and HVA in the striatum (P <0.05). Compared with the model group, the climbing time and the roller time of mice in the groups of dopas hydrazine tablets and the nalic acid were shortened (P <0.05), and DA, DOPAC and HVA in the striatum were increased (P <0.05 ). The numbers of spontaneous exercise in mice treated with doppler’s hydrazine tablets and high doses of nerve acid increased (P <0.05). Conclusion: Nerve acid can effectively improve the symptoms of dyskinesia in PD model mice, and its mechanism may be related to the increase of DA content in brain striatum.