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为了阐明籼稻(Oryza sativa L. spp. indica)、粳稻(O. sativa L. spp. japonica)对低温强光敏感性的差异,着重研究了低温强光下水稻类囊体膜脂不饱和度与叶黄素循环的变化。随着低温强光处理时间的延长,类囊体膜脂不饱和脂肪酸含量降低,饱和脂肪酸含量增加,因而膜脂不饱和指数(IUFA)下降。同时,叶黄素循环的关键酶——紫黄质脱环氧化酶(VDE)活性降低,叶黄素循环组分中紫黄质(V)含量增加,而单环氧玉米黄质(A)和玉米黄质(Z)的含量减少,表现为(A+Z)/(A+Z+V)比值下降。Arrhenius分析证明,VDE对低温和膜脂不饱和度都敏感。相关分析表明,类囊体IUFA分别与VDE活性、(A+Z)/(A+Z+V)和D1蛋白量呈显著的正相关。与粳稻9516相比,籼稻汕优63类囊体膜的IUFA较低,低温下类囊体膜脂流动性和稳定性较差,VDE活性和(A+Z)/(A+Z+V)比值较低。
In order to elucidate the difference of the sensitivity of O. sativa L. spp. Japonica to low temperature and high light in indica rice (Oryza sativa L. spp. Indica) and japonica rice (Oryza sativa L. spp. Japonica), the effects of low temperature and high light Lutein cycle changes. With the prolonging of cryogenic treatment time, the content of unsaturated fatty acids in the thylakoid membrane decreased and the content of saturated fatty acids increased. As a result, the IUFA decreased. At the same time, the key enzyme of lutein cycle - the activity of violaxanthin de-cyclooxygenase (VDE) decreased, the content of violaxanthin (V) increased in the lutein cycle component, while the content of monoepoxy zeaxanthin (A ) And zeaxanthin (Z) decreased as (A + Z) / (A + Z + V) decreased. Arrhenius analysis proves that VDE is sensitive to both low temperature and membrane lipid unsaturation. Correlation analysis showed that the IUFA of thylakoid showed a significant positive correlation with VDE activity, (A + Z) / (A + Z + V) and D1 protein, respectively. Compared with the japonica rice 9516, the IUFA of the Shanyou 63 thylakoid membrane was lower, and the fluidity and stability of the thylakoid membrane lipid were poor. The VDE activity and (A + Z) / (A + Z + V) The ratio is lower.