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植物防御素是一类富含半胱氨酸的小分子蛋白,在抗病反应中起着重要作用。本研究以结球白菜和拟南芥基因组序列为材料,利用生物信息学手段进行基因鉴定、染色体定位和进化分析。结果表明,结球白菜有11个防御素基因成员,数量少于拟南芥(15个),基因组DNA全长183～637 bp,开放阅读框全长183～363 bp;除结球白菜Bra029208序列外,其余防御素基因均有1个内含子;基因定位结果表明,一些基因成簇分布,推测由串联重复所致,拟南芥的3号、结球白菜的1、4、5和10号染色体均没有防御素基因的分布;进化分析结果表明,26个防御素成员可分为三大类,Ⅰ类有10条序列,Ⅱ类13条,Ⅲ类最少,仅3条。
Plant defensins are a class of cysteine-rich small molecule proteins that play an important role in disease resistance. In this study, the genomes of cabbage and Arabidopsis were used as materials, and bioinformatics methods were used to identify the genes, analyze the chromosomal location and evolutionary analysis. The results showed that there were eleven defensins genes in Brassica campestris, which were less than that in Arabidopsis thaliana (15). The genomic DNA was 183-637 bp in length and the full open reading frame was 183-363 bp in length. In addition to Bra029208 The other defensin genes had one intron. The results of gene mapping showed that some genes were clustered and deduced to be caused by tandem repeats. Arabidopsis thaliana 3, 1, 4, 5 and 10 There were no defensin genes in chromosomes. The results of phylogenetic analysis showed that 26 defensins could be divided into three groups, with 10 sequences in class Ⅰ, 13 in class Ⅱ, 3 in class Ⅲ and only 3 in total.