The technique of solution deposition was employed to prepare Li4Ti5O12 thin film using lithium acetate and TiO(C4H9)4 as starting materials. The structural and electrochemical properties of the thin films were studied by X-ray diffraction, cyclic voltammetry, galvanostatic charge-discharge experiments, and potential step technique. The results show that the thin film prepared by this method is of pure phase with a spinel framework structure. The capacity of the thin film annealed at 750 ℃ for 1 h is approximately 57 μA·h/(cm2·μm). The film possesses excellent cycling behavior with a 0.08% capacity loss per cycle after being cycled 50 times. Potential step technique shows that the average chemical diffusion coefficient of lithium ion in the thin film is approximately 4.5×10-11 cm2/s.