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本文报告将BCG64千道尔顿(kDa)蛋白基因克隆到pEMBL8及pEMBL9质粒中,再将质粒转化到大肠杆菌K-12株,获得重组基因的大肠杆菌称为大肠杆菌M 1103。64kDa抗原是分枝杆菌DNA在大肠杆菌中常见的表现型,它也存在于BCG菌体,可以由BCG的培养滤液里获得,这种抗原称为MbaA。将大肠杆菌M1103进行培养,收集菌体,提取MbaA,所得纯化物含有80%的64kDa蛋白及少量快移动成份。这种快移动成份能与抗MbaA单克隆抗体起反应,因此它似为64kDa蛋白的降解物,而不是其他无关的杂质。按荷兰乌德勒支大学的方法,对64kDa的DNA
In this paper, BCG64 kilodalton (kDa) protein gene was cloned into pEMBL8 and pEMBL9 plasmids, and then the plasmid was transformed into E. coli K-12 strain, the recombinant gene of E. coli is called E. coli M 1103.64kDa antigen is sub Mycobacterial DNA in E. coli common phenotype, it is also present in BCG bacteria, can be obtained from the BCG culture filtrate, this antigen is called MbaA. Escherichia coli M1103 was cultured, the cells were harvested, and MbaA was extracted. The resulting purified product contained 80% of 64 kDa protein and a small amount of fast moving component. This fast moving component reacts with the anti-MbaA monoclonal antibody, so it appears to be a 64 kDa protein degradation rather than other unrelated impurities. According to the method of University of Utrecht, Netherlands, a 64 kDa DNA was used