幽门螺杆菌(Hp)可导致胃炎、消化性溃疡、胃腺癌、粘膜相关淋巴组织淋巴瘤等多种疾病。Hp感染后胃粘膜中大量中性粒细胞和淋巴细胞浸润,并以Th1细胞免疫应答为主(Th1细胞极化),造成持续性组织损伤。为了确认以Th1细胞应答为主的胃粘膜免疫反应主要依赖于天然免疫应答,而非对Hp的抗原特异性识别。作者培养并收获Hp,裂解细菌,得到蛋白产物,并分离基因组DNA, Helicobacter pylori (Hp) can cause gastritis, peptic ulcer, gastric adenocarcinoma, mucosa-associated lymphoid tissue lymphoma and other diseases. Hp infection in gastric mucosa in a large number of neutrophils and lymphocytes infiltration, and Th1 cellular immune response (Th1 cell polarization), resulting in persistent tissue damage. In order to confirm that the gastric mucosal immune response mainly based on the Th1 cell response relies mainly on the innate immune response rather than the antigen-specific recognition of Hp. The authors cultivate and harvest Hp, lyse bacteria, get the protein product, and isolate the genomic DNA,