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目的建立高效液相色谱法测定人血浆和尿液中头孢拉宗浓度。方法以左氧氟沙星作为内标,血浆样品经5%高氯酸沉淀蛋白提取分离。结果头孢拉宗血浆和尿液在2～250μg.mL-1和5～500μg.mL-1线性良好,最低定量限分别为2,5μg.mL-1,提取回收率分别在68.8%～77.4%和89.7%～102.2%,准确度分别在95.4%～108.8%和86.5%～108.7%,血浆的日内、日间精密度(RSD)均≤3.9%,尿液的日内、日间精密度(RSD)均≤9.1%,结论建立的高效液相法专属性强,灵敏度高,操作简单,定量准确,实用性强,适合于头孢拉宗药代动力学研究。
Objective To establish a HPLC method for the determination of cefradrazone in human plasma and urine. Methods Levofloxacin was used as an internal standard, and plasma samples were separated by 5% perchloric acid precipitation protein. Results The cefradzu plasma and urine showed good linearity at 2 ~ 250μg.mL-1 and 5 ~ 500μg.mL-1 with the limits of quantification of 2,5μg.mL-1 and the recoveries of 68.8% -77.4% And 89.7% -102.2% respectively, with the accuracy of 95.4% -108.8% and 86.5% -108.7%, respectively. The intra-day and inter-day precision of RSD were all lower than 3.9% and the intra- and inter- ) Were less than or equal to 9.1% .Conclusion The established HPLC method has the advantages of high specificity, high sensitivity, simple operation, accurate quantitation and strong practicability. It is suitable for the study of cefradine pharmacokinetics.