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目的:比较不同工艺制备的美洲大蠊提取物抗肝损伤药效与主要成分含量差异。方法:采用水或乙醇分别回流法、闪式法和磁力搅拌法提取美洲大蠊,通过CCl4所致小鼠急性肝损伤模型考察药效,采用考马斯亮蓝法和茚三酮法测定美洲大蠊提取物中总肽及游离氨基酸含量,结合出膏率计算加权得分,考察不同提取工艺的差异。结果:水闪式提取物具有良好的对抗CCl4所致急性肝损伤小鼠血清MDA增多、抑制血清转氨酶增高、减轻肝组织病变的作用。各提取物中出膏率分别为16.64%,20.84%,16.19%,15.75%,19.53%,16.82%,总肽质量分数分别为0.82%,0.03%,0.96%,0.02%,0.64%,0.02%,游离氨基酸质量分数分别为1.99%,1.11%,2.12%,1.15%,2.10%,1.03%,综合评分分别为90.64,37.31,99.46,42.53,82.42,39.00。结论:美洲大蠊水闪式提取物具有良好的抗肝损伤药效与工艺指标评分。
OBJECTIVE: To compare the anti-hepatic injury efficacy and main components of Periplaneta americana extract prepared by different techniques. Methods: Periplaneta americana was extracted by water or ethanol reflux method, flash method and magnetic stirring method. The acute liver injury model induced by CCl4 was used to investigate the efficacy of the model. Coomassie brilliant blue method and ninhydrin method The total peptide and free amino acids in the extract were determined. The weighted scores were calculated by combining the ointment rates and the differences of the different extraction processes were investigated. Results: Water-flash extract had good anti-CCl4-induced acute liver injury in mice increased serum MDA, inhibit the increase of serum transaminases, reduce the role of liver lesions. The extraction rate of each extract was 16.64%, 20.84%, 16.19%, 15.75%, 19.53% and 16.82% respectively. The total peptide mass fractions were 0.82%, 0.03%, 0.96%, 0.02%, 0.64% and 0.02% , Free amino acids were 1.99%, 1.11%, 2.12%, 1.15%, 2.10%, 1.03%, respectively. The comprehensive scores were 90.64, 37.31, 99.46, 42.53, 82.42, 39.00 respectively. Conclusion: American cockroach water flash extract has good anti-liver injury efficacy and process index score.