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目的观察细胞外三磷酸腺苷(adenosine Triphosphate,ATP)对体外培养的U87人脑胶质瘤细胞增殖、侵袭和迁移能力的影响。方法采用MTT法检测不同浓度细胞外ATP对U87胶质瘤细胞增殖的影响;以相同条件下不加ATP作为对照组,以加入100μmol/L ATP作为处理组,采用Transwell细胞侵袭实验检测100μmol/L细胞外ATP对U87胶质瘤细胞侵袭能力的影响;用时间显微镜动态观察100μmol/L的ATP作用下U87胶质瘤细胞的迁移情况。结果高浓度(5 000μmol/L)细胞外ATP对U87胶质瘤细胞增殖有显著抑制作用,较低浓度(50、100、500μmol/L)细胞外ATP对U87胶质瘤细胞增殖无明显影响,5 000μmol/L ATP组与其他浓度组比较,差异有统计学意义(P<0.05);Transwell侵袭实验中,处理组细胞跨膜细胞数为(163.83±17.81)明显多于对照组(89.83±13.27)(P<0.01);迁移实验中,处理组细胞运动速度为(163.83±17.81)μm/h,对照组细胞运动速度为(89.83±13.27)μm/h,2组比较,差异有统计学意义(P<0.05)。结论 100μmol/L的细胞外ATP对体外培养的U87胶质瘤细胞的侵袭和迁移具有明显促进作用。
Objective To investigate the effect of extracellular adenosine triphosphate (ATP) on the proliferation, invasion and migration of U87 human glioma cells in vitro. Methods MTT assay was used to detect the effects of different concentrations of extracellular ATP on the proliferation of U87 glioma cells. Under the same conditions, without adding ATP as the control group, 100μmol / L ATP was added as the treatment group. Transwell invasion assay was used to detect the effects of 100μmol / L The effect of extracellular ATP on the invasion ability of U87 glioma cells was observed. The migration of U87 glioma cells was observed under the action of 100μmol / L ATP by time microscope. Results High concentrations of extracellular ATP (5 000 μmol / L) significantly inhibited the proliferation of U87 glioma cells. Low concentrations of extracellular ATP (50,100 and 500 μmol / L) had no effect on the proliferation of U87 glioma cells, Compared with other concentrations of 5 000 μmol / L ATP, the difference was statistically significant (P <0.05). In Transwell invasion assay, the number of transmembrane cells in the treated group was significantly higher than that in the control group (163.83 ± 17.81 vs 89.83 ± 13.27 ) (P <0.01). In the migration experiment, the velocity of the cells in the treatment group was (163.83 ± 17.81) μm / h and that in the control group was (89.83 ± 13.27) μm / h, the difference was statistically significant (P <0.05). Conclusion 100μmol / L extracellular ATP can significantly promote the invasion and migration of U87 glioma cells cultured in vitro.