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目的:探讨银杏内酯B对高胆固醇和apoE4致海马神经元凋亡的保护作用。方法:采用无血清培养基体外培养新生大鼠海马神经元,采用神经元特异性烯醇化酶免疫萤光进行鉴定。用160μg/mL银杏内酯B处理海马神经元16h,40μg/mL 25-OH-胆固醇和30μg/mL载脂蛋白E4继续处理24h。通过Hoechst试剂盒测定神经元的凋亡,实时定量RT-PCR测定bax和Bcl-2 mRNA的表达。结果:银杏内酯B抑制神经元凋亡,上调bcl-2 mRNA的表达,下调bax mRNA的表达。结论:银杏内酯B对高胆固醇和apoE4损伤海马神经元凋亡有保护作用,其机理可能与调节两种凋亡相关基因有关。
Objective: To investigate the protective effect of ginkgolide B on the apoptosis of hippocampal neurons induced by high cholesterol and apoE4. METHODS: Neonate rat hippocampal neurons were cultured in serum-free medium in vitro and identified by neuron-specific enolase immunofluorescence. Hippocampal neurons were treated with 160 μg/mL ginkgolide B for 16 h, 40 μg/mL 25-OH-cholesterol, and 30 μg/mL apolipoprotein E4 for 24 h. Neuron apoptosis was measured by Hoechst kit and real-time quantitative RT-PCR was used to determine the expression of bax and Bcl-2 mRNA. RESULTS: Ginkgolide B inhibited the apoptosis of neurons, up-regulated the expression of bcl-2 mRNA, and down-regulated the expression of bax mRNA. CONCLUSION: Ginkgolide B has protective effects on hippocampal neuronal apoptosis induced by high cholesterol and apoE4, and its mechanism may be related to the regulation of two apoptosis-related genes.