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目的探讨食品防腐剂苯甲酸钠对大鼠神经系统的损伤机制。方法选取40只健康SPF级SD大鼠,分为4组,每组10只,以苯甲酸钠低、中、高剂量组(1457、2914和4371 mg/kg)分别喂养,连续30天。处死后脑组织经前处理制成匀浆后,采用定磷比色法测定Na~+-K~+-ATP酶、Ca~(2+)-Mg~(2+)-ATP酶的活性,探讨苯甲酸钠对大鼠脑线粒体的损害;以苄胺为底物,经环己烷提取,于242 nm下测定吸光度,继而测定出单胺氧化酶(MAO)活性;匀浆上清液中加入巯基显色剂进行比色定量,于412 nm下测其吸光度从而测定乙酰胆碱酯酶(AChE)的活力;匀浆上清液中加入1.00 mL碱性羟胺,加入0.5 mL 4 moL/L盐酸及0.50 mL10%三氯化铁,于530 nm处比色,得出乙酰胆碱(ACh)的含量。结果苯甲酸钠影响大鼠体重的增长和一般生长发育,可降低大鼠脑组织中ATP酶、MAO及AChE活性,增加ACh含量。结论苯甲酸钠在一定程度上能造成大鼠脑组织神经系统的损害。
Objective To investigate the mechanism of food preservative sodium benzoate on the nervous system in rats. Methods Forty Sprague-Dawley (SD) SD rats were randomly divided into 4 groups (10 rats in each group), which were fed with low, medium and high doses of sodium benzoate (1457, 2914 and 4371 mg / kg respectively) for 30 consecutive days. After sacrificed, the brain tissue was prepared and homogenized, and the activity of Na ~ + -K ~ + -ATPase and Ca ~ (2 +) - Mg ~ (2 +) - ATPase Sodium benzoate on rat brain mitochondrial damage; benzylamine as a substrate, extracted with cyclohexane, absorbance was measured at 242 nm, and then measured monoamine oxidase (MAO) activity; homogenate supernatant was added thiol reagent And the absorbance was measured at 412 nm to determine the activity of acetylcholinesterase (AChE); 1.00 mL of basic hydroxylamine was added to the homogenate supernatant, 0.5 mL of 4 mol / L hydrochloric acid and 0.50 mL of 10% trichloro Iron, colorimetric at 530 nm, acetylcholine (ACh) content. Results Sodium benzoate increased body weight gain and general growth and development in rats, and decreased ATPase, MAO and AChE activities and increased ACh content in rat brain. Conclusion Sodium benzoate to a certain extent, can cause damage to the nervous system of rat brain tissue.