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目的 观察小鼠糖尿病脑淀粉样 β肽 (Aβ)和内质网Aβ结合蛋白 (ERAB)的变化 ,以了解糖尿病神经元退变的可能机制并观察淀粉样 β蛋白前体 (APP) 17肽的作用。 方法 用链脲佐菌素诱发糖尿病小鼠模型 ,并用APP17肽保护。 4周后 ,取脑 ,做APP17肽、Aβ 1～ 40、Aβ 1～ 42、Aβ 1～ 16及ERAB免疫组化染色。结果 正常小鼠海马内表达APP17肽、Aβ 1～ 40、Aβ 1～ 42、Aβ 1～ 16及ERAB的神经元数目少 ,染色淡 ,糖尿病小鼠海马内上述各种神经元的染色数目及染色程度均比正常小鼠明显增加 (P <0 .0 5 ) ,用APP17肽保护后 ,上述各种神经元染色数目及程度与正常小鼠接近。结论 糖尿病小鼠海马神经元表达Aβ和ERAB比正常小鼠明显增加 ,这可能是糖尿病脑病海马神经元退变的机制之一。APP17肽可使糖尿病小鼠Aβ及ERAB表达恢复正常 ,提示APP17肽有调节Aβ代谢和改善神经元退变的功能。
Objective To observe the changes of cerebral amyloid β peptide (ERβ) and endoplasmic reticulum Aβ - binding protein (ERAB) in diabetic mice and to explore the possible mechanism of degeneration of diabetic neurons and to observe the effects of amyloid β - protein precursor (APP) 17 peptide effect. Methods Diabetic mice were induced with streptozotocin and protected with APP17 peptide. After 4 weeks, brain was taken for immunohistochemical staining of APP17 peptide, Aβ 1-40, Aβ 1-42, Aβ 1-16 and ERAB. Results The number of neurons expressing APP17 peptide, Aβ1-40, Aβ1-42, Aβ1-16 and ERAB in normal mice was less than that in normal mice. The numbers and staining of above neurons in hippocampus of mice with stained light and diabetic (P <0.05). After being protected with APP17 peptide, the numbers and degree of staining of the above various neurons were similar to those in normal mice. Conclusion The expression of Aβ and ERAB in hippocampal neurons of diabetic mice is significantly higher than that of normal mice, which may be one of the mechanisms of degeneration of hippocampal neurons in diabetic encephalopathy. APP17 peptide can restore normal expression of Aβ and ERAB in diabetic mice, suggesting that APP17 peptide can regulate Aβ metabolism and improve neuronal degeneration.