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目的 研究酪醇使肝癌细胞ＩＩ相解毒酶ＮＡＤ(Ｐ)Ｈ :醌氧化还原酶 1(ＮＱ0 1)诱导表达和细胞增殖的抑制以及两者之间的关系。方法 肝癌细胞ＳＭＭＣ 772 1接种后 2 4ｈ经 β 酪醇处理 2 4ｈ ,分别测定酶活性、ｍＲＮＡ表达和细胞增殖情况。ＮＱ0 1酶活性采用微孔板直接测定
Objective To investigate the effect of tyrosol on the expression of cytosolic detoxification enzyme NAD(P)H : quinone oxidoreductase 1 (NQO 1) and the inhibition of cell proliferation in hepatoma cells and their relationship. Methods The activity, mRNA expression and cell proliferation of the liver cancer cells SMMC 772 1 were determined by β-tyrosol treatment 24 h after 24 h. NQ0 1 enzyme activity was measured directly on the microplate