论文部分内容阅读
目的分析口岸输入性甲型H1N1流感病毒神经氨酸酶(NA)基因特征及变异趋势,及早发现变异株。方法选取广东口岸入境人员中检出的64份输入性甲型H1N1流感阳性样本,对其病毒NA基因进行核苷酸序列测定。与2009年甲型H1N1流感代表株A/California/04/2009/H1N1的NA基因序列进行对比,对甲型H1N1流感病毒的NA基因进化树、核苷酸序列同源性、氨基酸序列的变异及蛋白分子结构变化等进行分析。结果 NA基因进化树分为3个分支,其中2009年、2010年病毒聚集成簇位于分支1,2011年病毒则与部分2010年检出的病毒位于较远距离的分支2和3。检出的1例病毒NA氨基酸的抗原决定簇上发生Y155H的变异,部分病毒NA蛋白在42位点上新增了一个糖基化位点,在68、386位点出现了糖基化位点的缺失。氨基酸G41R、V106I、V241I、N248D、I365T、N369K等位点的变异较为显著,但未见耐奥司他韦病毒的出现。结论甲型H1N1流感病毒NA基因已发生一定程度的变异。随着突变的不断积累,病毒极可能会通过改变其抗原性和耐药性,从而引起新的流行。
Objective To analyze the characteristics and variation trend of neuraminidase (NA) gene of imported influenza A (H1N1) virus in port and find the mutant early. Methods Sixty-four positive samples of imported Influenza A (H1N1) from Guangdong port entry workers were selected and their NA genes were sequenced. Compared with the NA gene sequence of A / California / 04/2009 / H1N1 strain of Influenza A (H1N1) 2009, the phylogenetic tree, nucleotide sequence homology and amino acid sequence variation of NA gene of H1N1 influenza virus Protein molecular structure changes were analyzed. Results The NA gene phylogenetic tree was divided into three branches, of which 2009 and 2010 clustered in cluster 1 in clusters. In 2011, the virus was located in branches 2 and 3 located far away from some of the viruses detected in 2010. A mutation of Y155H was detected in the antigenic determinant of one amino acid of NA, some of which added a new glycosylation site at position 42 and a glycosylation site at position 68, 386 The lack of. Variations of amino acids G41R, V106I, V241I, N248D, I365T, N369K and other loci were significant, but there was no emergence of resistance to oseltamivir. Conclusion The NA gene of Influenza A (H1N1) virus has been mutated to some extent. As mutations continue to accumulate, the virus most likely causes a new epidemic by altering its antigenicity and drug resistance.